NK cell ADCC recognition assays using normal human IgG (control), 3BNC117, or PGT121 at a T:E of 10 for 6 hours followed by flow cytometry analysis of NK cell function. (A) Representative recognition assay plots. (B) HIV-specific ADCC enhances responses to both infected CD4+ T cells and macrophages. Data summaries for NK cell responses (%CD107a + %TNF-α) from six independent experiments (n=9 biological replicates). These raw values were not corrected for background recognition of uninfected cells. (C) Total NK cell ADCC responses to infected macrophages are significantly lower compared to ADCC responses to CD4+ T cells. “%CD107a + %TNF-α” values are initially corrected for background recognition of uninfected targets and normalized to target cell infection frequencies, followed by corrections of recognition in IgG conditions. Shown are data from six independent experiments (n=9 biological replicates). (D) Data summaries for the quality of the NK cell response from six independent experiments (n=9 biological replicates). Values are corrected for background recognition of uninfected target cells. Ratios of CD107a versus TNF-α production were calculated as described in the STAR Methods. Statistical analysis for (B-D): paired t tests, **p<0.01, ***p<0.001, ****p<0.0001. See also Fig. S5.