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. 2021 Sep 28;7(9):e08087. doi: 10.1016/j.heliyon.2021.e08087

Figure 3.

Figure 3

Pressure stability of bLf. Pressure stability of apo- and holo-bLf incubated or not with 2 M GnHCl was monitored by intrinsic fluorescence emission. The measured values of spectral center of mass after return to atmospheric pressure are denoted as “return”. The protein was diluted in standard buffer to a final concentration of 500 μg/mL. Data were obtained from three independent experiments and plotted as mean ± SD. Differences in means compared to control were not significant for holo- and apo-bLf up to 0.8 kbar, apo-bLf [GnHCl] 2 M up to 0.4 kbar and holo-bLf [GnHCl] 2 M up to 1 kbar. Differences in spectral center of mass upon return to atmospheric pressure was not significant for holo-bLf and holo-bLf [GnHCl] 2 M compared to control condition at 0 kbar (p > 0.05). For apo-bLf and apo-bLf [GnHCl] 2 M the return had a significant difference of spectral center of mass in relation to the control (p ≤ 0.05 and p ≤ 0.001, respectively).