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Cytotoxicity of bLf. In order to verify the cytotoxic effect of bLf (apo and holo) treatment on Vero cells, different concentrations of this protein were used (0.25, 0.5, 1 and 2 mg/mL). The negative control represents cells not treated with bLf and the positive control cells treated with 0.1% Triton X-100. Data points represent the average of three independent measurements. Only the difference in mean of Triton X-100 compared to control was significant (p ≤ 0.001).
