REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Casirivimab	Laboratory of Hans-Martin Jäck	N/A
Imdevimab	Laboratory of Hans-Martin Jäck	N/A
Bamlanivimab	Laboratory of Hans-Martin Jäck	N/A
Etesevimab	Laboratory of Hans-Martin Jäck	N/A
hIgG	Laboratory of Hans-Martin Jäck	N/A
Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488	Thermo Fisher Scientific	Cat# A-11013; RRID: AB_2534080
Anti-VSV-G antibody (I1, produced from CRL-2700 mouse hybridoma cells)	ATCC	Cat# CRL-2700; RRID: CVCL_G654
Bacterial and virus strains
VSV∗ΔG-FLuc	Laboratory of Gert Zimmer	N/A
One Shot OmniMAX 2 T1R Chemically Competent E. coli	Thermo Fisher Scientific	Cat# C854003
Biological samples
Patient Plasma (SI 15)	Laboratory of Martin Sebastian Winkler	N/A
Patient Plasma (SI 18)	Laboratory of Martin Sebastian Winkler	N/A
Patient Plasma (SI 22)	Laboratory of Martin Sebastian Winkler	N/A
Patient Plasma (SI 23)	Laboratory of Martin Sebastian Winkler	N/A
Patient Plasma (SI 24)	Laboratory of Martin Sebastian Winkler	N/A
Patient Plasma (SI 27)	Laboratory of Martin Sebastian Winkler	N/A
Patient Plasma (SI 33)	Laboratory of Martin Sebastian Winkler	N/A
Patient Plasma (SI 51)	Laboratory of Martin Sebastian Winkler	N/A
Vaccinee Serum (ID4844)	Laboratory of Georg M.N. Behrens	N/A
Vaccinee Serum (ID4846)	Laboratory of Georg M.N. Behrens	N/A
Vaccinee Serum (ID4847)	Laboratory of Georg M.N. Behrens	N/A
Vaccinee Serum (ID4848)	Laboratory of Georg M.N. Behrens	N/A
Vaccinee Serum (ID4849)	Laboratory of Georg M.N. Behrens	N/A
Vaccinee Serum (ID4863)	Laboratory of Georg M.N. Behrens	N/A
Vaccinee Serum (ID4864)	Laboratory of Georg M.N. Behrens	N/A
Vaccinee Serum (ID4865)	Laboratory of Georg M.N. Behrens	N/A
Vaccinee Serum (ID4866)	Laboratory of Georg M.N. Behrens	N/A
Vaccinee Serum (ID4867)	Laboratory of Georg M.N. Behrens	N/A
Vaccinee Serum (ID4868)	Laboratory of Georg M.N. Behrens	N/A
Vaccinee Serum (ID4872)	Laboratory of Georg M.N. Behrens	N/A
Vaccinee Serum (ID4874)	Laboratory of Georg M.N. Behrens	N/A
Vaccinee Serum (ID4876)	Laboratory of Georg M.N. Behrens	N/A
Vaccinee Serum (ID4877)	Laboratory of Georg M.N. Behrens	N/A
Chemicals, peptides, and recombinant proteins
Soluble human ACE2 (sol-hACE2-Fc)	Laboratory of Stefan Pöhlmann	N/A
Critical commercial assays
Beetle-Juice Kit	PJK	Cat# 102511
Experimental models: Cell lines
293T	DSMZ	Cat# ACC-635; RRID: CVCL_0063
A549 (ACE2)	Laboratory of Stefan Pöhlmann	N/A
BHK-21	Laboratory of Georg Herrler	ATCC Cat# CCL-10; RRID:CVCL_1915
Caco-2	Laboratory of Stefan Pöhlmann	ATCC Cat# HTB-37; RRID: CVCL_0025
Calu-3	Laboratory of Stephan Ludwig	ATCC Cat# HTB-55; RRID: CVCL_0609
Vero76	Laboratory of Andrea Maisner	ATCC Cat# CRL-1586; RRID: CVCL_0574
Oligonucleotides
SARS-2-S (BamHI) F (AAGGCCGGATCCG CCACCATGTTCGTGTTTCTGGTGCTGC)	Sigma-Aldrich	N/A
SARS-2-SΔ18 (XbaI) R (AAGGCCTCTAG ACTACTTGCAGCAGCTGCCACAG)	Sigma-Aldrich	N/A
SARS-2-S (T19R) F (GTGAACCTGCGCAC AAGAACCCAGCTGCCTCCAGC)	Sigma-Aldrich	N/A
SARS-2-S (T19R) R (GTTCTTGTGCGCAG GTTCACACACTGGCTGGAC)	Sigma-Aldrich	N/A
SARS-2-S (E142D) F (CCTTCCTGGACGTC TACTATCACAAGAAC)	Sigma-Aldrich	N/A
SARS-2-S (E142D) R (TAGTAGACGTCCAGGAAGGGGTCGTTG CAGAAC)	Sigma-Aldrich	N/A
SARS-2-S (E142D/E156G/F157Δ/R158Δ) Hyb F (GTTCTGCAACGACCCCTTCCTG GACGTCTACTATCACAAGAACAACAA GAGCTGG)	Sigma-Aldrich	N/A
SARS-2-S (E142D/E156G/F157Δ/R158Δ) Hyb R (CAGTTGTTGGCGCTGCTGTACA CCCCGCTTTCCATCCAGCTCTTGTTGTT CTTGTGATAG)	Sigma-Aldrich	N/A
SARS-2-S (E156G/F157Δ/R158Δ) F (TGGAAAGCGGGGTGTACAGCA GCGCCAACAACTGC)	Sigma-Aldrich	N/A
SARS-2-S (E156G/F157Δ/R158Δ) R (GCTGTACACCCCGCTTTCCAT CCAGCTCTTGTTG)	Sigma-Aldrich	N/A
SARS-2-S (L452R) F (TACAATTACCG GTACCGGCTGTTCCGGAAGTC)	Sigma-Aldrich	N/A
SARS-2-S (L452R) R (CAGCCGGTACc GGTAATTGTAGTTGCCGCCGAC)	Sigma-Aldrich	N/A
SARS-2-S (L452R/T478K) Hyb F (ACAATTACCGGTACCGGCTGTTCCG GAAGTCCAATCTGAAGCCCTTCGAG CGGGACATC)	Sigma-Aldrich	N/A
SARS-2-S (L452R/T478K) Hyb R (TTACAAGGTTTGCTGCCGGCCTG ATAGATCTCGGTGGAGATGTCCC GCTCGAAGGGCTTC)	Sigma-Aldrich	N/A
SARS-2-S (T478K) F (CCGGCAGCA AACCTTGTAACGGCGTGGAAGGCTTC)	Sigma-Aldrich	N/A
SARS-2-S (T478K) R (GTTACAAGGTTT GCTGCCGGCCTGATAGATCTCG)	Sigma-Aldrich	N/A
SARS-2-S (P681R) F (CAAACAGCCGC AGACGGGCCAGATCTGTG)	Sigma-Aldrich	N/A
SARS-2-S (P681R) R (GCCCGTCTGC GGCTGTTTGTCTGTGTCTG)	Sigma-Aldrich	N/A
SARS-2-S (D950N) F (AAGCTGCAGAA CGTGGTCAACCAGAATGCCCAGG)	Sigma-Aldrich	N/A
SARS-2-S (D950N) R (TGACCACGTTCT GCAGCTTTCCCAGGGCGCTTGC)	Sigma-Aldrich	N/A
SARS-2-S Seq-01 (CAAGATCTACAGCAAGCACACC)	Sigma-Aldrich	N/A
SARS-2-S Seq-02 (GTCGGCGGCAACTACAATTAC)	Sigma-Aldrich	N/A
SARS-2-S Seq-03 (CTGTCTGATCGGAGCCGAGCAC)	Sigma-Aldrich	N/A
SARS-2-S Seq-04 (TGAGATGATCGCCCAGTACAC)	Sigma-Aldrich	N/A
SARS-2-S Seq-05 (GCCATCTGCCACGACGGCAAAG)	Sigma-Aldrich	N/A
pCG1 F (CCTGGGCAACGTGCTGGT)	Sigma-Aldrich	N/A
pCG1 R (GTCAGATGCTCAAGGGGCTTCA)	Sigma-Aldrich	N/A
Recombinant DNA
Plasmid: pCG1	Laboratory of Roberto Cattaneo	N/A
Plasmid: pCAGGS-VSV-G	Laboratory of Stefan Pöhlmann	N/A
Plasmid: pCAGGS-DsRed	Laboratory of Stefan Pöhlmann	N/A
Plasmid: pCG1-SARS-2-SΔ18 (D614G), codon-optimized	Laboratory of Stefan Pöhlmann	N/A
Plasmid: pCG1-SARS-2-SΔ18 (B.1.351), codon-optimized	Laboratory of Stefan Pöhlmann	N/A
Plasmid: pCG1-SARS-2-SΔ18 (B.1.617.2), codon-optimized	Laboratory of Stefan Pöhlmann	N/A
Plasmid: pCG1-solACE2-Fc	Laboratory of Stefan Pöhlmann	N/A
Software and algorithms
Hidex Sense Microplate Reader Software	Hidex Deutschland Vertrieb GmbH	https://www.hidex.de
YASARA (version 19.1.27)	YASARA Biosciences GmbH	http://www.yasara.org
Adobe Photoshop CS5 Extended (version 12.0 × 32)	Adobe	https://www.adobe.com/
GraphPad Prism (version 8.3.0(538))	GraphPad Software	https://www.graphpad.com/
ZEN imaging software	Carl Zeiss	https://www.zeiss.com/corporate/int/home.html
Microsoft Office Standard 2010 (version 14.0.7232.5000)	Microsoft Corporation	https://www.microsoft.com/microsoft-365
Other
Complex of SARS-CoV-2 receptor binding domain with the Fab fragments of two neutralizing antibodies (PDB: 6XDG)	Hansen et al., 2020	https://www.rcsb.org/structure/6XDG
SARS-CoV 2 Spike Protein bound to LY-CoV555 (PDB: 7L3N)	Jones et al., 2020	https://www.rcsb.org/structure/7L3N
Molecular basis for a potent human neutralizing antibody targeting SARS-CoV-2 RBD (PDB: 7C01)	Shi et al., 2020	https://www.rcsb.org/structure/7C01
Distinct conformational states of SARS-CoV-2 spike protein (PDB: 6XR8)	Cai et al., 2020	https://www.rcsb.org/structure/6XR8