Fig. 4.

Cordycepin ameliorated lysosomal dysfunction in senescent cells. (A) Acid phosphatase activity was detected after H₂O₂ and cordycepin treatment. The data were analyzed using Student’s t‐test and are shown as the mean ± SD. *P < 0.05. (B) The control group and senescent fibroblasts were treated with 40 μm cordycepin for 48 h, and the WB indicates the expression of lysosomal‐associated membrane protein 2 (LAMP2) and representative enzyme cathepsin B (CTSB) on the lysosomal membrane. (C) The lysosomal status was probed with a LysoTracker Red DND‐99 kit after senescent cells were treated with cordycepin, and representative images of three independent replicates are shown. Scale bars = 10 μm. (D) WB detected the expression of senescence‐related protein as well as LAMP2 and CTSB after treatment with cordycepin alone and with NH₄Cl. Data were analyzed using Student’s t‐test and are presented as the means ± SD. *P < 0.05, **P < 0.01.