Table 1.
Antibodies for Immunoblotting
| Antibody | MW | Vendor | Cat. number | Dilution |
|---|---|---|---|---|
| Primary antibodies | ||||
| AMPKα | 62 | Cell Signaling Technology, Danvers, MA | 23A3 | 1:1000 |
| H3ac | 17 | Millipore, Burlington, MA | 06-599 | 1:500 |
| H3K27ac | 15 | Abcam, Cambridge, MA | ab177178 | 1:1000 |
| H3K27me3 | 15 | Sigma-Aldrich, St. Louis, MO | 07-449 | 1:500 |
| H3K9ac | 17 | PMT BIOLABS, Chicago, IL | PMT-156 | 1:2000 |
| H3K9ac | 17 | Active Motif, Carlsbad, CA | 39918 | 1:500 |
| H3K9me3 | 17 | Abcam, Cambridge, MA | ab8898 | 1:1000 |
| HDAC5 | 124 | Cohesion Biosciences, London, UK | CPA2445 | 1:500 |
| HDAC4 | 119 | Boster Biological Technology, Pleasanton, CA | A00971-1 | 1:500 |
| PPARα | 52 | Abcam, Cambridge, MA | ab24509 | 1:500 |
| PPARγ | 53 | Cell Signaling Technology, Danvers, MA | 81B8 | 1:1000 |
| pAMPKα | 63 | Cell Signaling Technology, Danvers, MA | 2535 | 1:1000 |
| pHDAC5 | 124 | Affinity Biosciences, Pottstown, PA | ABIN6267700 | 1:500 |
| β-actin | 42 | Sigma-Aldrich, St. Louis, MO | A5441 | 1:5000 |
| Secondary antibodies | ||||
| Anti-mouse | Jackson Immunoresearch Laboratories, West Grove, PA | 115-035-003 | 1:10,000 | |
| Anti-rabbit | Jackson Immunoresearch Laboratories, West Grove, PA | 111-035003 | 1:10,000 | |
MW, molecular weight.