Fig. 2.

Gene profile analysis of SNc in MPTP-induced PD mice treated with celastrol. a Hierarchical clustered heatmap of gene expression profiles for celastrol or vehicle treatment in SNc under the condition of MPTP injection. b Heatmap of DEGs of MPTP-received mice treated with celastrol or vehicle. c KEGG analysis highlights the DEGs of celastrol treatment compared with vehicle in MPTP-recieved mice, upregulated genes are colored in red, downregulated genes are colored in blue. (P < 0.05 with unpaired two-tailed Student's t-tests). d Gene Ontology enrichment was based on DEGs that have a P value smaller than 0.05. Enrichment analysis for Gene Ontology terms among the genes of a gene–trait correlation module was performed using Metascape. e Volcano plot displays DEGs of celastrol treatment compared with vehicle in MPTP-received mice. Significantly altered genes are colored in blue, insignificantly altered genes are colored in red. f Venn diagram of overlapping significantly changed genes (±1.2 fold, P < 0.05). The top ten overlapping genes are presented in diagram. g Representative immunoblots and quantification of relative protein levels in SNc. Data are mean ± s.e.m.; n = 5 biologically independent animals; *P < 0.05, **P < 0.01, and ***P < 0.001. h Protein–protein interaction network identified among Nrf2, NLRP3 and Caspase-1 using STRING database. i Human SNc mRNA correlations analysis by Gene network.