Fig. 3.

Neuroprotective effects of celastrol in PD are Nrf2 dependent. a Representative TH staining of SNc and STR, unbiased stereological counts of TH⁺ neurons in SNc and the quantity of TH⁺ striatal fiber density in STR of WT and Nrf2-KO mice. Scale bar, 400 μm for SNc, 2 mm for STR. b Representative diagram of gait test of WT and Nrf2-KO mice treated with celastrol or vehicle. c Representative immunoblots and quantification of TH, DAT in SNc or STR levels of WT and Nrf2-KO mice. Data are mean ± s.e.m.; n = 9. d Time to traverse beam apparatus, time to descend pole, hind-limb clasping reflex score, fall latency from an accelerating rotarod. Data are mean ± s.e.m.; n = 9. Two-way ANOVA followed by Tukey's post hoc test. *P < 0.05, **P < 0.01, and ***P < 0.001. ns, not significant.