Fig. 1.

HDAC inhibitor mediated EMT in SW13 cells is not influenced by changes in cellular iron availability. (A) EMT-like changes in actin (green) morphology induced by treatment with 2 nM FK228 are not altered by iron chelation (FK228 + DFO) or iron supplementation (FK228 + Hemin). (B) Increased expression of the mesenchymal marker, vimentin by FK228 treatment is not influenced by co-treatment with DFO or hemin. (C) Increased mRNA expression of the mesenchymal markers SMARCA2, TGFβ1, and SNAI1 following HDAC inhibitor treatment were not influence by iron chelation. (D) The mRNA expression of matrix metalloproteinase enzymes MMP2 and MMP9 was also increased by HDAC inhibitor treatment and unaffected by iron chelation. (E) Increased intracellular iron accumulation following HDAC inhibitor mediated EMT (FK228) were consistent with levels observed following iron supplementation (Hemin). Images were taken using a 40X objective lens. Data are presented as mean ± SEM. * Denotes significant difference compared to control, p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)