Fig. 2.

IRP mRNA binding activity is increased and ferroportin expression is decreased following HDAC inhibitor mediated EMT in SW13 cells. Spontaneous (A) and total (B) mRNA binding activity and expression of the IRP target proteins TFRC and ferroportin (C) in SW13 cells that were treated with DMSO (Control) or with 2 nM FK228 for 24 h were measured by gel-shift assay and Western blot, respectively. GAPDH was used as a loading control. Data are presented as mean ± SEM. * Denotes significant difference compared to control, p < 0.05.