Fig. 3.

Both iron chelation and iron supplementation reduce cell viability in HDAC inhibitor treated SW13 cells. (A) Images and (B) quantitation of EdU-positive cells in SW13 cells following treatment with DMSO (Control) or 2 nM FK228 treatment for 24 h and then co-treatment with 50 μM DFO (FK228 + DFO) or 40 μM hemin (FK228 + Hemin) for another 24 h. (C) Percent cell viability was measured by fluorometric assay. Images were taken through an 4X objective lens. Data are presented as mean ± SEM. *Denotes statistical significance, p < 0.05.