Preparation of uridine-supplemented complete medium

Reagent	Final concentration	Amount
DMEM w/ 4.5 g/L glucose, sodium pyruvate, and L-glutamine	-	869 mL
Uridine (Acros Organics, Cat#140770250)	50 mg/L	1 mL (from 50 mg/mL stock)
Penicillin Streptomycin solution	1×	10 mL (from 100× stock)
GlutaMAX Supplement	1×	10 mL (from 100× stock)
MEM Non-essential Amino Acids	1×	10 mL (from 100× stock)
Fetal Bovine Serum (FBS)	10%	100 mL
Total	n/a	1000 mL

Medium can be stored at 4°C for up to 1 month.