Preparation of SIMR selection medium

Reagent	Final concentration	Amount
DMEM w/ 4.5 g/L glucose, sodium pyruvate, and L-glutamine	-	870 mL
Penicillin Streptomycin solution	1×	10 mL (from 100× stock)
GlutaMAX Supplement	1×	10 mL (from 100× stock)
MEM Non-essential Amino Acids	1×	10 mL (from 100× stock)
Dialyzed Fetal Bovine Serum (FBS) (Thermo Fisher Scientific, Cat#26400-044)	10%	100 mL
Total	n/a	1000 mL

Medium can be stored at 4°C for up to 1 month.