Skip to main content
. 2021 Sep 28;2(4):100850. doi: 10.1016/j.xpro.2021.100850
REAGENT or RESOURCE SOURCE IDENTIFIER
Chemicals, peptides, and recombinant proteins

DMEM w/ 4.5 g/L glucose, sodium pyruvate, and L-glutamine Corning Cat#10013CM
DMEM, no glucose Thermo Fisher Scientific Cat#11966025
Fetal Bovine Serum Omega Scientific Cat#FB-11
Fetal Bovine Serum, dialyzed, US origin Thermo Fisher Scientific Cat#26400-044
Penicillin-Streptomycin Solution, 100× Corning Cat#30-002-CI
GlutaMAX Supplement Thermo Fisher Scientific Cat#35050061
MEM Non-Essential Amino Acids Solution (100X) Thermo Fisher Scientific Cat#11140050
Uridine Acros Organics Cat#140770250
D-(+)-Galactose Millipore Sigma Cat#G5388-100G
Dulbecco’s Phosphate-Buffered Salt Solution 1X Corning Cat#21031CV
Sodium Pyruvate (100 mM) Corning Cat#25000Cl
Dulbecco's Phosphate-Buffered Salt Solution, w/ Calcium and Magnesium Thermo Fisher Scientific Cat#14040133
StemPro Accutase Cell Dissociation Reagent Thermo Fisher Scientific Cat#A1110501
16% Formaldehyde Thermo Fisher Scientific Cat#28906
Crystal Violet Fisher Chemical Cat#C581-25
Methanol Fisher Chemical Cat#A412-4

Critical commercial assays

Qproteome Mitochondria Isolation Kit QIAGEN Cat#37612

Experimental models: Cell lines

143 BTK– osteosarcoma ρ0; human, female (Patananan et al., 2020) N/A
Primary dermal fibroblast normal ρ0; human, male, neonatal (Patananan et al., 2020) N/A
HEK293T dsRed; human, female Miyata et al., 2014 N/A

Other

Breadboard - Full-Size (Bare) SparkFun Cat#12615
Solenoid - 5V SparkFun Cat#ROB-11015
Momentary Pushbutton Switch - 12mm Square SparkFun Cat#COM-09190
Hook-up Stranded Wire - Black (22 AWG) SparkFun Cat#PRT-08867
JST Jumper 2 Wire Assembly SparkFun Cat#PRT-09914
Jumper Wire Kit - 140pcs SparkFun Cat#PRT-00124
Power Supply Mini Board Futurelec Cat#MINIPOWER
35W Single Output Switching Power Supply MEAN WELL Cat#RS-35-12
Mains Power Cord, NEMA 5-15P to IEC 60320 C13, 2.3 m, 10 A, 125 VAC, Black Volex Cat#1725010B1
Push-In Plugs with Wire Leads, 2.1 mm End ID, 12V DC McMaster-Carr Cat#8320N111
Spanner Wrench for SM1-Threaded Retaining Rings Thorlabs Cat# SPW602
Externally SM1-Threaded Plug Thorlabs Cat#SM1PL
SM1-Threaded 30 mm Cage Plate, 0.50" Thick, 2 Retaining Rings, 8–32 Tap Thorlabs Cat#CP02T
Cage Assembly Rod, 3" Long, Ø6 mm Thorlabs Cat#ER3
SM1-Threaded 30 mm Cage Plate, 0.35" Thick, 2 Retaining Rings, 8–32 Tap Thorlabs Cat#CP02
Custom Aluminum Washer University of California, Los Angeles, Machine Shop N/A
Custom Aluminum Case University of California, Los Angeles, Machine Shop N/A
SYLGARD™ 184 Silicone Elastomer Kit 1.1 KG Kit Dow Corning Cat#1673921
Polypropylene Desiccator with Stopcock Thermo Fisher Scientific Cat#53100250
230 mm Ceramic Metal Desiccator Plate Thermo Fisher Scientific Cat#53120230
10 mm Hole punch McMaster-Carr Cat#3418A1
25 mm Hole punch McMaster-Carr Cat#3418A25
Expanded Plasma Cleaner Harrick Plasma Cat#PDC-001
12–Well 3.0 μm Transparent PET Membrane Cell Culture Inserts Corning Cat#353181
26 G Blunt-ended needle VWR Cat#89134-164
3 mL Luer-Lok Syringe BD Biosciences Cat#309657
High-vacuum grease Dow Corning Cat#1597418
Cloning cylinders Fisher Scientific Cat# 09-552-21