| Chemicals, peptides, and recombinant proteins |
|
| DMEM w/ 4.5 g/L glucose, sodium pyruvate, and L-glutamine |
Corning |
Cat#10013CM |
| DMEM, no glucose |
Thermo Fisher Scientific |
Cat#11966025 |
| Fetal Bovine Serum |
Omega Scientific |
Cat#FB-11 |
| Fetal Bovine Serum, dialyzed, US origin |
Thermo Fisher Scientific |
Cat#26400-044 |
| Penicillin-Streptomycin Solution, 100× |
Corning |
Cat#30-002-CI |
| GlutaMAX Supplement |
Thermo Fisher Scientific |
Cat#35050061 |
| MEM Non-Essential Amino Acids Solution (100X) |
Thermo Fisher Scientific |
Cat#11140050 |
| Uridine |
Acros Organics |
Cat#140770250 |
| D-(+)-Galactose |
Millipore Sigma |
Cat#G5388-100G |
| Dulbecco’s Phosphate-Buffered Salt Solution 1X |
Corning |
Cat#21031CV |
| Sodium Pyruvate (100 mM) |
Corning |
Cat#25000Cl |
| Dulbecco's Phosphate-Buffered Salt Solution, w/ Calcium and Magnesium |
Thermo Fisher Scientific |
Cat#14040133 |
| StemPro Accutase Cell Dissociation Reagent |
Thermo Fisher Scientific |
Cat#A1110501 |
| 16% Formaldehyde |
Thermo Fisher Scientific |
Cat#28906 |
| Crystal Violet |
Fisher Chemical |
Cat#C581-25 |
| Methanol |
Fisher Chemical |
Cat#A412-4 |
|
| Critical commercial assays |
|
| Qproteome Mitochondria Isolation Kit |
QIAGEN |
Cat#37612 |
|
| Experimental models: Cell lines |
|
| 143 BTK– osteosarcoma ρ0; human, female |
(Patananan et al., 2020) |
N/A |
| Primary dermal fibroblast normal ρ0; human, male, neonatal |
(Patananan et al., 2020) |
N/A |
| HEK293T dsRed; human, female |
Miyata et al., 2014 |
N/A |
|
| Other |
|
| Breadboard - Full-Size (Bare) |
SparkFun |
Cat#12615 |
| Solenoid - 5V |
SparkFun |
Cat#ROB-11015 |
| Momentary Pushbutton Switch - 12mm Square |
SparkFun |
Cat#COM-09190 |
| Hook-up Stranded Wire - Black (22 AWG) |
SparkFun |
Cat#PRT-08867 |
| JST Jumper 2 Wire Assembly |
SparkFun |
Cat#PRT-09914 |
| Jumper Wire Kit - 140pcs |
SparkFun |
Cat#PRT-00124 |
| Power Supply Mini Board |
Futurelec |
Cat#MINIPOWER |
| 35W Single Output Switching Power Supply |
MEAN WELL |
Cat#RS-35-12 |
| Mains Power Cord, NEMA 5-15P to IEC 60320 C13, 2.3 m, 10 A, 125 VAC, Black |
Volex |
Cat#1725010B1 |
| Push-In Plugs with Wire Leads, 2.1 mm End ID, 12V DC |
McMaster-Carr |
Cat#8320N111 |
| Spanner Wrench for SM1-Threaded Retaining Rings |
Thorlabs |
Cat# SPW602 |
| Externally SM1-Threaded Plug |
Thorlabs |
Cat#SM1PL |
| SM1-Threaded 30 mm Cage Plate, 0.50" Thick, 2 Retaining Rings, 8–32 Tap |
Thorlabs |
Cat#CP02T |
| Cage Assembly Rod, 3" Long, Ø6 mm |
Thorlabs |
Cat#ER3 |
| SM1-Threaded 30 mm Cage Plate, 0.35" Thick, 2 Retaining Rings, 8–32 Tap |
Thorlabs |
Cat#CP02 |
| Custom Aluminum Washer |
University of California, Los Angeles, Machine Shop |
N/A |
| Custom Aluminum Case |
University of California, Los Angeles, Machine Shop |
N/A |
| SYLGARD™ 184 Silicone Elastomer Kit 1.1 KG Kit |
Dow Corning |
Cat#1673921 |
| Polypropylene Desiccator with Stopcock |
Thermo Fisher Scientific |
Cat#53100250 |
| 230 mm Ceramic Metal Desiccator Plate |
Thermo Fisher Scientific |
Cat#53120230 |
| 10 mm Hole punch |
McMaster-Carr |
Cat#3418A1 |
| 25 mm Hole punch |
McMaster-Carr |
Cat#3418A25 |
| Expanded Plasma Cleaner |
Harrick Plasma |
Cat#PDC-001 |
| 12–Well 3.0 μm Transparent PET Membrane Cell Culture Inserts |
Corning |
Cat#353181 |
| 26 G Blunt-ended needle |
VWR |
Cat#89134-164 |
| 3 mL Luer-Lok Syringe |
BD Biosciences |
Cat#309657 |
| High-vacuum grease |
Dow Corning |
Cat#1597418 |
| Cloning cylinders |
Fisher Scientific |
Cat# 09-552-21 |