Fig. 1.

Schematic representation of the RC-PCR technology used in EasySeq™ SARS-CoV-2 WGS kit (NimaGen, Nijmegen, The Netherlands). The protocol consists of one single PCR-like reaction consisting of 2 steps. The schematic is adapted from Kieser et al., (Kieser et al., 2020) A. Two types of oligo's are present, 1) the universal tail which includes a Unique Dual Index (UDI), sequence adapter, and universal sequence. 2) The RC primer which contains an extension blocker, universal sequence, and the reverse complement of the SARS-CoV-2 genomic target sequence. B. The universal sequences anneal and form a SARS-CoV-2 specific PCR primer. C – E. A regular PCR in which the SARS-CoV-2 specific amplicons are created. F. The final amplicons are ready to sequence on an illumina sequencer.