Fig. 3.

Inhibition of PrP^Sc-seeding activity by TC-5RW in vitro. A RT-QuIC assay of prion-seeding activity of brain homogenates from 263 K-infected hamsters in the presence of different amounts of TC-5RW ranging from 0–50 μg/mL. B Western blotting of PK-resistant PrP^Sc of 263 K-infected hamster sPMCA products (+ PMCA, right panel) and samples without sPMCA (− PMCA, left panel) in the presence of different amounts of TC-5RW (0–50 μg/mL). C RT-QuIC assay of prion-seeding activity of skin homogenates from 263 K-infected Tg7 mice expressing hamster PrP^C in the presence of different amounts of TC-5RW ranging from 0–50 μg/mL. PBH, positive brain homogenate. No seed in A and C RT-QuIC without brain homogenate seeds as a control to exclude spontaneous seeding activity