Fig. 4.

Measurement of C1q levels in C1q KO, C3 KO, Crry KO, and C7 KO mouse serum by quantitative sandwich ELISA. (A) Serum C1q levels were measured in C1q KO mice; no C1q was detected at 6 or 12 months. (B-C) Measurement of serum C1q levels in B C3 KO and C Crry KO mice at 6 and 12 months of age; no significant differences in serum C1q levels were observed between 6 and 12 months (P > 0.05). (D) Measurement of serum C1q levels in C7 KO mice; a significant decrease was identified at 12 months compared to 6 months of age (P < 0.01). (E) Serum C1q levels in C3 KO, C7 KO, and C1q KO were significantly reduced relative to WT and Crry KO at 6 months. At 12 months, serum C1q was only significantly reduced in C7 KO and C1q KO mice compared to WT and Crry KO mice. There were no significant differences between WT and Crry KO mice. * = P < 0.05, ** = P < 0.01, *** = P < 0.001