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. 2021 May 8;58(9):4237–4250. doi: 10.1007/s12035-021-02403-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — The results of immunofluorescence and TUNEL staining in the hippocampus of pups (N = 6, PND7). a. Double immunofluorescence with antibodies against NeuN (green) and phospho-cAMP response element-binding protein (p-CREB) (red) demonstrated the expression of p-CREB on hippocampal neurons. b. Double immunofluorescence with antibodies against NeuN (green) and cleaved caspase-3 (blue) demonstrated the expression of cleaved caspase-3 on hippocampal neurons. c. The neural apoptosis in the hippocampus, as shown by TUNEL assay (TUNEL, green; DAPI, blue). The merged images showed overlapping localization. d–f. Quantitative analysis of the relative expression level expression of p-CREB, cleaved caspase-3 and TUNEL⁺. Scale bar = 500 μm. TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling