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. 2021 Oct 2;7:272. doi: 10.1038/s41420-021-00659-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a miR-539 expression in 20 normal tissues and 54 cancer tissues was analyzed based on GEO (GSE30454). HCT116 cells transfected with miR-539 mimic or miR-NC and the SW480 cells transfected with miR-539 inhibitor or inhibitor-NC for 24 h were collected, and the transfected cells were used in the subsequent assays. b The expression level of miR-539 was determined in HCT116 and SW480 cells after transfecting. c CCK-8 assays were conducted to examine the effects of miRNA-539 mimic and miR-539 inhibitor transfection on the proliferation of SW480 and HCT116 cells. d Representative colony formation assays were conducted to examine the effects of miRNA-539 mimic and miR-539 inhibitor transfection on the proliferation of SW480 and HCT116 cells. e The transfected cells were subjected to transmission electron microscopy and calculated the rate of shrunken mitochondria. Representative pictures are shown. Arrows, nuclei; arrowheads, mitochondria. f The transfected cells were labeled with ROS and Lipid ROS probes, the levels of ROS and Lipid ROS were detected by flow cytometry and analyzed statistically. Data are presented as the mean ± SD. **p < 0.01; ***p < 0.001; ****p < 0.0001.