ISA estimates for a, fraction of palmitate synthesized following [U-13C6]glucose addition, b, fraction of palmitate synthesized following [5-13C]glutamine addition, c, fraction of palmitate contributed by [5-13C]glutamine, d, fraction of palmitate contributed by [U-13C5]glutamine, and e, fraction of palmitate contributed by [U-13C6]glucose as functions of growth rate in EV-HMECs under variable concentrations of EGF.
Analyzed MIDs were averages of n = 3 wells, error bars represent 95% confidence intervals, and results are representative of two replicate experiments. P-values reflect the likelihood that the regression line slopes differ from zero and were determined using the extra sum-of-squares F-test.