Table 1.
Proposed functions of proteins of the rub BGC in Streptomyces sp. CB02414
| Protein | Size (aa) | Proposed function | Homologs | |
|---|---|---|---|---|
| Protein/organism | Accession no. (similarity/identity %) | |||
| Rub(-1) | 258 | AfsR/SARP family regulator | MoaR2/Streptomyces sp. S4.7 | QHY99565.1 (54/68) |
| RubA1 | 237 | Regulator | HrbR3 | BAJ07866.1 (54/66) |
| RubB1 | 235 | Oxidoreductase | HrbS | BAJ07858.1 (48/61) |
| RubC | 279 | Type II toxin-antitoxin chaperone | HigA/Pseudomonas fluorescens F113 | AEV65067.1 (51/65) |
| RubD1 | 489 | Oxygenase | HrbR | BAJ07857.1 (70/78) |
| RubE | 108 | Cyclase | HrbB | BAJ07841.1 (70/78) |
| RubF1 | 424 | Ketosynthase (KSα) | HrbC | BAJ07842.1 (80/89) |
| RubF2 | 406 | Chain length factor (KSβ) | HrbE | BAJ07844.1 (69/80) |
| RubF3 | 85 | Acyl carrier protein | HrbM | BAJ07852.1 (59/67) |
| RubG | 261 | Ketoreductase | HrbO | BAJ07854.1 (80/87) |
| RubH | 361 | Aromatase | HrbP | BAJ07855.1 (67/79) |
| RubI1 | 678 | Hydroxylase | HrbG | BAJ07846.1 (68/78) |
| RubB2 | 256 | Oxidoreductase | HrbS | BAJ07858.1 (71/81) |
| RubJ | 522 | Decarboxylase | JadN/Streptomyces venezuelae | AAK01934.1 (86/92) |
| RubK | 71 | Acyl-CoA carboxylase subunit | C7987_1162/Streptomyces venezuelae | PWW46500.1 (69/77) |
| RubD2 | 322 | Oxygenase | HrbH | BAJ07847.1 (77/85) |
| RubL | 414 | Transporter | HrbT | BAJ07859.1 (59/70) |
| RubI2 | 206 | Hydroxylase | HrbW | BAJ07862.1 (52/68) |
| RubD3 | 221 | Antibiotic biosynthesis monooxygenase | ADL21_13530/Streptomyces albus | KWT61349.1 (36/49) |
| RubM1 | 228 | Methyltransferase | HrbF | BAJ07845.1 (44/58) |
| RubA2 | 201 | TetR family regulator | BetI/Streptomyces sp. Go-475 | AXE89736.1 (76/84) |
| RubB3 | 275 | Oxidoreductase | HrbS | BAJ07858.1 (24/41) |
| RubN1 | 404 | Cytochrome P450 | NcmG/Saccharothrix syringae | ARS01480.1 (54/66) |
| RubM2 | 288 | Methyltransferase | DWB77_01891 | AYG79773.1 (48/58) |
| RubO | 260 | Alpha/beta hydrolase | DWB77_01892 | AYG79774.1 (47/64) |
| RubA3 | 212 | Lys family regulator | DWB77_01893 | AYG79775.1 (58/69) |
| RubP1 | 254 | Hypothetical protein | DWB77_01894 | AYG79776.1 (59/71) |
| RubP2 | 373 | Hypothetical protein | DWB77_01895 | AYG79777.1 (70/78) |
| RubB4 | 488 | Oxidoreductase | DWB77_01896 | AYG79778.1 (57/65) |
| RubM3 | 291 | Methyltransferase | DWB77_01897 | AYG79779.1 (80/88) |
| RubP3 | 567 | GH3 auxin-responsive promoter | DWB77_01898 | AYG79780.1 (45/59) |
| RubP4 | 209 | Hypothetical protein | DWB77_01899 | AYG79781.1 (66/77) |
| RubQ | 431 | Acyltransferase | PauY24/Streptomyces sp. YN86 | AIE54244.1 (44/57) |
| RubR | 71 | Ferredoxin | HbmFdx/Streptomyces hygroscopicus | AAY28236.1 (50/62) |
| RubN2 | 403 | Cytochrome P450 | SceD/Streptomyces sp. SD85 | ANH11399.1 (47/63) |
| RubA4 | 225 | TetR regulatory protein | AbmC/Streptomyces koyangensis | AVI57423.1 (50/62) |
| RubL2 | 493 | MFS transporter | AbmD/Streptomyces koyangensis | AVI57425.1 (46/65) |
| RubM4 | 345 | O-Methyltransferase | HrbU | BAJ07860.1 (60/74) |
| RubS | 585 | Acetyl-/propionyl-CoA carboxylase subunit alpha | JadJ/Streptomyces venezuelae | AAD37851.1 (86/91) |
| Rub(+ 1) | 407 | Methionine adenosyltransferase | SLA_0973/Streptomyces laurentii | BAU81924.1 (92/95) |
aThe hrb homologous proteins are from Streptomyces sp. 2238-SVT4
bThe DWB77_01891 to DWB77_01899 proteins are from Streptomyces hundungensis