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. 2021 Oct 2;52:126. doi: 10.1186/s13567-021-00997-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Representative colorimetric results for positive and negative reactions. Positives taken from quantitative loop-mediated isothermal amplification (qLAMP) reactions run with 10 000 copies of DNA per reaction and negatives taken from qLAMP reactions without DNA. Kmt1 primers were used to detect PM, rsmL primers were used to detect MH and lolB primers were used to detect HS. All samples were imaged at 60 min. Images were collected using an Epson Perfection V800 Photo scanner and the background was whitened using the ImageJ brightness/contrast setting. PM, Pasteurella multocida; MH, Mannheimia haemolytica; HS, Histophilus somni.