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. 2021 Oct 2;19:412. doi: 10.1186/s12967-021-03075-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Characterization of hsa_circ_0005092 and hsa_circ_0002647. a The RT-PCR product of the hsa_circ_0005092 is verified by sanger sequencing. b qRT–PCR analysis for the expression of hsa_circ_0005092 and its host gene after actinomycin D treatment for 0 h, 3 h, 6 h, 9 h, and 12 h in SNU-1 cells. c RNase R resistance assay for has_circ_0005092 in SNU-1 and MKN-45 cells. d The RT-PCR product of the hsa_circ_0002647 is verified by sanger sequencing. e qRT–PCR analysis for the expression of hsa_circ_0002647 and its host gene after actinomycin D treatment. f RNase R resistance assay for has_circ_0002647 in SNU-1 and MKN-45 cells. IPO7, importin 7; EHMT1, euchromatic histone lysine methyltransferase 1