FIG. 7.

p43 induces a T3-dependent increase in mitochondrial translation. In organello translation experiments were performed as described for Fig. 6 in the presence of [³⁵S]methionine and incubated at 37°C for 120 min. Translation experiments were stopped by cooling on ice. Mitochondria were collected by centrifugation, and mitochondrial proteins were TCA precipitated on Whatman filter 41, washed five times with 5% TCA, and then washed twice with ethanol. Radioactivity was measured by liquid scintillation counting. [³⁵S]methionine incorporation into mitochondrial proteins was expressed as a percentage of control values. Data are presented as the means ± the SEM of five separate experiments. When indicated, 10⁻⁸ M T3 was added to the incubation medium.