FIG. 9.
Stable p43 overexpression in QM7 cells increases COX I mRNA levels without affecting mt-TFA expression. QM7 cells were cultured in a T3-depleted medium according to the method of Samuels et al. (40), and, when indicated, 10−8 M T3 was added to the incubation medium. (A) Northern blot analysis with total RNA extracted from the control or from p43-overexpressing QM7 cells demonstrated that p43 overexpression increases COX I mRNA levels in the presence of exogenous T3; amounts of RNA loaded in each lane were assessed by using S26 mRNA as a control. (B) p43 overexpression in QM7 cells and/or exogenous T3 do not influence mt-TFA protein. Western blot experiments were performed with an antiserum raised against mt-TFA. Proteins (100 μg) were loaded in each lane.