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. Author manuscript; available in PMC: 2022 Jul 21.
Published in final edited form as: ACS Chem Neurosci. 2021 Jul 2;12(14):2693–2704. doi: 10.1021/acschemneuro.1c00345

Figure 5.

Figure 5.

Constellation pharmacology indicates that 3 blocks ACh signaling in primary cultures of SCG neurons. SCG cells were treated with ACh (300 μM), 3 (0.2, 2 and 20 μM, and TxID (1 μM). A) Average number of neurons with ACh response blocked by 3 (20 μM) (n = 2). B) Representative individual SCG neurons responding to ACh. The y-axis indicates intracellular [Ca2+], reflected in the normalized min/max fluorescence ratio of 340 nM/380 nM from the Fura-2-AM Ca2+ indicator. The x-axis is time (min), where ACh (1 mM) is repeatedly pulsed (arrows), with incubation of 3 and TxID. Inset figures show the bright-field image of the corresponding cell (cross-sectional area in μm2).