Skip to main content
. Author manuscript; available in PMC: 2022 Jul 21.
Published in final edited form as: ACS Chem Neurosci. 2021 Jul 2;12(14):2693–2704. doi: 10.1021/acschemneuro.1c00345

Figure 7.

Figure 7.

Compound 3 is antinociceptive in the formalin test. Male adult mice (n = 8 per group) were pretreated with 3 (5 mg/kg, 20 mg/kg, 30 mg/kg, i.p), vehicle (5 mL/kg, i.p.) or the positive control duloxetine (10 mg/kg, 10 mL/kg, i.p.). Formalin (5% in saline) was injected into the left hind paw pad after dosing; nociceptive behaviors were recorded for 0-35 min. Phase I represents the sum of time spent on nociceptive behavior in the first 5 min immediately after formalin injection while Phase II denotes the response period 20-35 min after injection. Each animal is represented by a circle, and the average SEM is shown with error bars. Significance in comparison to vehicle: *p<0.05, **p<0.01, ****p<0.0001. Compound 3 inhibited nociceptive behaviors in Phase I at doses 5 mg/kg (p<0.05), 20 mg/kg (p<0.05), and 30 mg/kg (p = 0.06) and in Phase II at 30 mg/kg (p = 0.06).