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. 2021 Sep 23;148(18):dev199695. doi: 10.1242/dev.199695

Fig. 1.

Fig. 1.

Strategy for endogenously tagging Celsr1, Fz6 and Vangl2. (A) Schematic of the proposed localization of the core transmembrane PCP proteins at the cellular and junctional level. (B,D,F) Diagram of Celsr1-3xGFP (B), Fz6-3xGFP (D) and tdTomato-Vangl2 (F) fusion proteins illustrating domain structure and the position of insertion. (C,E) Targeting arm design for the C-terminal Celsr1-3xGFP (C) and Fz6-3xGFP (E) insertion. The Celsr1 and Fz6 stop codons targeted by the sgRNA and the C-terminal exons of the Celsr1 (blue) and Fz6 (magenta) genomic region are shown. (G) Targeting arm design for N-terminal fusion of tdTomato-Vangl2. The Vangl2 start codon targeted by the sgRNA and the N-terminal exons (orange) of the Vangl2 genomic region are shown.