Fig. 5.

Fz6-3xGFP localization is highly dynamic during cell rearrangements and divisions yet the global axis of asymmetry is maintained. (A) Still frames from live imaging of Fz6-3xGFP homozygous E15.5 skin explants at 0, 2, 4 and 6 h time points. Circular histograms below show quantification of Fz6-3xGFP polarity across the entire field of view. Note the orientation and magnitude of the polarity is maintained over the time course. (B) Still frames of Fz6-3xGFP live imaging during cell divisions and rearrangements. Top panels: a colored star indicates cells in mitosis and a pair of dots after cytokinesis. Bottom panels: same still frames as above with polarity nematics overlaid. Note that Fz6-3xGFP polarity within individual cells is not fixed through time, rather polarity changes in magnitude and orientation, especially with division. (C) Representative example of Fz6-3xGFP during cell division. Polarity nematics are overlaid on the dividing cell (dashed outline), and neighboring cells are darkened to emphasize Fz6-3xGFP in the dividing cell. Note, in this example daughter cells share the same axis of asymmetry as the mother cells before mitosis. Scale bars: 40 µm (A); 20 µm (B,C).