Table 1.

Composition of the CSFM and the Medium 199

CSFM		Medium 199 with 5% FBS
Components/100 ml	Amount	Components/100 ml	Amount
Minimum Essential Medium Eagle	1.04 g	Medium 199	1.47 g
Sodium Bicarbonate	0.22 g	Sodium bicarbonate	0.22 g
MEM non-essential amino acid solution (100x)	1 ml	Heat-inactivated FBS	5 ml
HEPES	40 mM	Adenosine (10 Mm)	0.1 mM
MEM amino acids (50× )	2 ml	HEPES	40 mM
L. glutamine	1 mM	L. glutamine	1 mM
Hemin	0.5 µg/ml	Gentamicin	100 µg/ml
BME vitamins 100× solution	1 ml	Hemin	0.5 µg/ml
D-glucose	0.45 g