FIGURE 5.

As₄S₄ changed the levels of p53, PD‐L1 and miR‐34a‐5p in A549/DDP cells. (a) Effect of As₄S₄ (1.5 μM) on p53 and PD‐L1 protein expression in A549/DDP cells treated for 0, 12, 24 and 48 h. (b) p53 protein quantification of the western blot results in A549/DDP cells treated with As₄S₄ (1.5 μM) for 0, 12, 24 and 48 h. Protein levels were normalized to the GAPDH levels and are shown as fold increase or decrease relative to the levels for the control strain. (c) PD‐L1 protein quantification of the western blot results in A549/DDP cells treated with As₄S₄ (1.5 μM) for 0, 12, 24 and 48 h. (d) miR‐34a‐5p levels of the real‐time qPCR results in A549/DDP cells treated with As₄S₄ (1.5 μM) for 0, 12, 24 and 48 h. (e) miR‐34a‐3p levels of the real‐time qPCR results in A549/DDP cells treated with As₄S₄ (1.5 μM) for 0, 12, 24 and 48 h. (f) Effect of As₄S₄ (0, 2.0, 2.5, 3.0 μM) on p53 and PD‐L1 protein expression in H1299/DDP cells treated for 48 h. (g) PD‐L1 protein quantification of the western blot results in H1299/DDP cells treated with As₄S₄ (0, 2.0, 2.5, 3.0 μM) for 48 h. (h) miR‐34a‐5p levels of the real‐time qPCR results in H1299/DDP cells treated with As₄S₄ (0, 2.0, 2.5, 3.0 μM) for 48 h. (i) miR‐34a‐3p levels of the real‐time qPCR results in H1299/DDP cells treated with As₄S₄ (0, 2.0, 2.5, 3.0 μM) for 48 h. Data represents the mean ± SD of three independent experiments (*p < 0.05; **p < 0.01; ***p < 0.001)