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. 2021 Sep 25;2021:6256369. doi: 10.1155/2021/6256369

Figure 2.

Figure 2

E2F1 activates BAIAP2-AS1 expression in HCC cells. (a) E2F1 binding site prediction in the BAIAP2-AS1 promoter region by the use of JASPAR. (b, c) The expression pattern of BAIAP2-AS1 in HCC by analyzing TCGA datasets. (d) The correlation between E2F1 and BAIAP2-AS1 expressions in HCC samples using TCGA datasets. (e) Expression level of BAIAP2-AS1 was detected in four HCC cells using RT-qPCR and Western blot. (f) The expression of BAIAP2-AS1 and E2F1 in HepG2 and PLC5 cells transfected with si-E2F1-1 or si-E2F1-2. (g) Overexpression of E2F1 resulted in the upregulation of BAIAP2-AS1. (h) ChIP-qPCR analysis of E2F1 occupancy in the BAIAP2-AS1 promoter in HepG2 and PLC5 cells. (i) Construction of the luciferase reporter vector. (j) Dual-luciferase reporter assays were applied to examine the combination between BAIAP2-AS1 and E2F1. ∗∗p < 0.01 and ∗∗∗p < 0.001.