Figure 6.

Profile of immune cells in the blood of mice vaccinated with PRAK-03202. A-G) Flow cytometric analysis of the lymphocyte subset percentage of A) CD4⁺, B) CD8⁺, C) CD11b⁺, D) CD11c⁺, E) Ly6G⁺, F) B cells, and G) NK cells in blood samples were performed by using the indicated antibodies at a 1:100 dilution. Error bars indicate means ± S.E.M. H) Sera from immunized mice were collected at various time points (0, 21, 61, 76, 86, 97, and 110 days). Total IgG response was evaluated using ELISA with a 1:5000 dilution of HRP-conjugated secondary antibodies (A4416, Sigma, USA). An IgG response was observed even after 110 days, suggesting long-term efficacy of PRAK-03202. Error bars indicate SEM.