Final Concentration	for 40 mL
30% formamide	12 mL formamide
5X SSC	10 mL 20X SSC
9 mM citric acid (pH 6.0)	360 μL 1 M citric acid, pH 6.0
0.1% Tween 20	400 μL 10% Tween 20
50 μg/mL heparin	200 μL 10 mg/mL heparin
1X Denhardt’s	400 μL 100X Denhardt’s solution
10% dextran sulfate	8 mL 50% dextran sulfate
	Adjust to 40 mL with H2O, store −20°C