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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: Cold Spring Harb Protoc. 2021 Oct 1;2021(10):pdb.prot105635. doi: 10.1101/pdb.prot105635
Final Concentration for 4 L
1 M maleic acid 464.4 g maleic acid (Sigma M0375)
1.5 mM NaCl 350.6 g NaCl
pH to 7.2 with ~ 280 g NaOH
Adjust to 4L with H2O