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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: Cold Spring Harb Protoc. 2021 Oct 1;2021(10):pdb.prot105635. doi: 10.1101/pdb.prot105635
Final Concentration for 40 mL
30% formamide 12 mL formamide
5X SSC 10 mL 20X SSC
9 mM citric acid (pH 6.0) 360 μL 1 M citric acid, pH 6.0
0.1% Tween 20 400 μL 10% Tween 20
50 μg/mL heparin 200 μL 10 mg/mL heparin
Adjust to 40 mL with H2O