| Final Concentration | for 100 mL |
|---|---|
| 1X PBS (immuno) or 0.5X SSC (ISH) | 10 mL 10X PBS + 81 mL H2O or 2.5 mL 20X SSC + 88.5 mL H2O |
| 5% formamide | 5 mL formamide |
| 1.2% peroxide | 4 mL of 30% peroxide |
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