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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: Cold Spring Harb Protoc. 2021 Oct 1;2021(10):pdb.prot105635. doi: 10.1101/pdb.prot105635
Final Concentration for 500 mL
10% BMB Blocking Agent 50 g BMB Blocking Agent (Sigma 11096176001)
1X MAB 50 mL 10X MAB
Adjust to 500 mL with H2O, mix with heat until dissolved
Store at this 10% concentration at −20°C in 50 mL aliquots
Dilute to 2% with 1X MAB on staining day