| Final Concentration | for 4 L |
|---|---|
| 92.625% Ethanol | 3.9 L 95% Ethanol |
| 25 mM Tris, pH 8 | 100 mL 1 M Tris, pH 8 |
| 1.25 mM EDTA | 10 mL 0.5 M EDTA |
| Adjust to 4 L with H2O |
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