Final Concentration	for 3 L
50% formamide	1.5 L formamide
5X SSC	750 mL 20X SSC
1 mg/ml Torula RNA	3 g Torula RNA Type IX (Sigma R3629)
100 μg/mL heparin	0.3 g heparin (Sigma H3393)
1X Denhart’s solution	30 mL 100X Denhardt’s solution
0.1% Tween 20	3 mL Tween 20
0.1% CHAPS	3 g CHAPS (Sigma C3023)
10 mM EDTA	11.167 g EDTA
	Adjust to 3 L with H2O, check that pH ~ 7.5, and store −20°C