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. Author manuscript; available in PMC: 2022 Nov 1.
Published in final edited form as: J Immunol Methods. 2021 Aug 28;498:113132. doi: 10.1016/j.jim.2021.113132

Table 3A.

Performance characteristics of RNA isolated from the filtrates stored at −80°C or shipped to the GEM Core at Ambient (Room) Temperature.

S.no Volume (cc)a A260/280 Ratiob Nanodrop based total RNA (μg)c Qubit based total RNA (μg)d 18S rRNA copies/ μg RNAe TGFpl copies/ μg RNAe has-miR-26a copies/ μg RNAe
80°C RT 80°C RT 80°C RT −80°C RT −80°C RT −80°C RT
1 50 1.85 2.00 2.94 2.57 1.01 1.07 2.36E+08 2.70E+08 1.42E+03 2.87E+03 2.82E+06 2.36E+06
2 50 2.06 2.13 0.83 0.79 0.42 0.44 1.32E+09 8.86E+08 9.30E+03 3.64E+03 9.57E+05 1.16E+06
3 50 2.04 2.01 1.83 1.59 1.39 1.32 1.92E+07 3.02E+07 1.68E+03 6.62E+02 1.00E+07 1.08E+07
Median 50 2.04 2.01 1.83 1.59 1.01 1.07 2.36E+08 2.70E+08 1.68E+03 2.87E+03 2.82E+06 2.36E+06
25th Percentile 50 1.85 2.00 0.83 0.79 0.42 0.44 1.92E+07 3.02E+07 1.42E+03 6.62E+02 9.57E+05 1.16E+06
75th Percentile 50 2.06 2.13 2.94 2.57 1.39 1.32 1.32E+09 8.86E+08 9.30E+03 3.64E+03 1.00E+07 1.08E+07
Wilcoxon P value f 0.50 0.25 >0.9999 >0.9999 0.75 0.75

Urine samples were processed using the ZR Urine RNA Isolation Kit™ and the filtrates were stored either at −80°C or shipped overnight to the GEM Core. Total RNA was isolated from the filtrates and analyzed.

a

The volume of urine samples processed.

b

A260/280 ratio measured using the NanoDrop® ND-1000 spectrophotometer.

c

The amount of RNA measured using the NanoDrop® ND-1000 spectrophotometer.

d

The amount of total RNA measured using the Qubit fluorometer.

e

Absolute copy numbers of 18S rRNA, TGF-β1 mRNA, and hsa-miR-26a miRNA were measured using customized RT-qPCR assays using gene specific oligonucleotide pairs and TaqMan probes on 7500 Fast Real-Time PCR System.

f

P values derived using Wilcoxon matched pairs signed-ranks test.