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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Genet Med. 2021 Jun 10;23(10):1912–1921. doi: 10.1038/s41436-021-01222-w

Figure 3. Truncated SHANK1 proteins abolished Homer1 binding.

Figure 3.

(A) Schematic figures denote SHANK1 c.3314del (p.Gly1105fs) and c.3355G>T (p.Glu1119*) variants and resulting SHANK1 protein truncations. (B) HA-SHANK1 (WT, Gly1105fs, or Glu1119*), GKAP-myc and Homer1b were co-transfected in HEK293 cells as indicated in the figures. GKAP-myc and Homer1b in the immunoprecipitates with HA antibody were analyzed by immunoblotting. (C) The immunoprecipitated HA-SHANK1 (WT, Gly1105fs, or Glu1119*) beads from HEK293 cells were incubated with cultured rat cortical neuron lysates for pulldown assays. Endogenous Homer1 binding with the immunoprecipitated HA-SHANK1 was analyzed by immunoblotting.