. Author manuscript; available in PMC: 2022 Oct 25.

Published in final edited form as: J Pharm Biomed Anal. 2021 Aug 27;205:114335. doi: 10.1016/j.jpba.2021.114335

Table 3.

Stability studies of 3-hydroxypentanoic acid and 3-oxopentanoic acid in human plasma.

			3-Hydroxypentanoic Acid Quality Control Nominal Concentrations (μg/mL)		3-Oxopentanoic Acid Quality Control Nominal Concentrations (μg/mL)

Stability Assessment (Conditions)	Parameter	n	0.225 (LQC)	4.00 (HQC)	0.450 (LQC)	8.00 (HQC)

Autosampler (30 hours)	Mean (μg/mL)	6	0.25	3.90	0.47	8.3
	Accuracy (%RE)^a	6	9.9	−2.5	4.2	3.4
	Precision (%RSD)^b	6	2.1	4.1	5.7	3.9
Benchtop (6 hours)	Mean (μg/mL)	6	0.23	3.8	0.45	8.4
	Accuracy (%RE)^a	6	2.7	−5.0	−0.4	4.5
	Precision (%RSD)^b	6	2.2	4.2	2.5	4.8
Freeze Thaw (3 cycles)	Mean (μg/mL)	6	0.22	3.8	0.40	8.5
	Accuracy (%RE)^a	6	−1.3	−4.8	−11	6.8
	Precision (%RSD)^b	6	1.7	2.9	2.1	2.7
Long Term (27 days at −80 °C)	Mean (μg/mL)	6	0.24	3.6	0.42	8.7
	Accuracy (%RE)^a	6	7.8	−8.9	−6.6	9.3
	Precision (%RSD)^b	6	1.7	2.4	8.7	6.8

= Percent Relative Error

= Percent Relative Standard Deviation