KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and virus strains | ||
| BL21 Competent Cells | NEB | Cat#C2527H |
| DH10Bac Competent Cells | invitrogen | Cat#10361012 |
| Experimental Models: Cell Lines | ||
| Human: HEK293 GnTI− cells | ATCC | CRL-3022 |
| Insect: Sf9 cells | Expression Systems | Cat#94-001S |
| Chemicals, peptides, and recombinant proteins | ||
| Double-knot toxin (DkTx) | This paper | N/A |
| Resiniferatoxin (RTX) | Sigma | Cat#R8756 |
| N-Methyl-D-glucamine (NMDG) | Sigma | Cat#M2004 |
| QX 314 chloride | Tocris | Cat#2313 |
| YO-PRO-1 iodide | invitrogen | Cat#Y3603 |
| Freestyle 293 Expression Medium | gibco | Cat#12338-018 |
| Insect cell culture media | Expression Systems | Cat#96-001-01 |
| FETAL BOVINE SERUM | PEAK | Cat#PS-FB1 |
| BOVINDE CALF SERUM | HyClone | Cat#SH30072.03 |
| Deposited data | ||
| minimal TRPV1, apo | This paper | EMD-23136; PDB-7L2P |
| minimal TRPV1, DkTx pre-bound | This paper | EMD-23139; PDB-7L2S |
| minimal TRPV1, DkTx singly-bound | This paper | EMD-23161 |
| minimal TRPV1, DkTx, pre-open | This paper | EMD-23138; PDB-7L2R |
| minimal TRPV1, DkTx, partial open | This paper | EMD-23140; PDB-7L2T |
| minimal TRPV1, DkTx, open | This paper | EMD-23141; PDB-7L2U |
| minimal TRPV1, RTX/NMDG a | This paper | EMD-23143; PDB-7L2W |
| minimal TRPV1, RTX/NMDG b | This paper | EMD-23142; PDB-7L2V |
| minimal TRPV1, RTX/NMDG c | This paper | EMD-23144; PDB-7L2X |
| minimal TRPV1, 1 perturbed PI | This paper | EMD-24084; PDB-7MZ6 |
| minimal TRPV1, 1 partially bound RTX | This paper | EMD-24086; PDB-7MZ9 |
| minimal TRPV1, 4 partially bound RTX | This paper | EMD-24085; PDB-7MZ7 |
| minimal TRPV1, 2 bound RTX in adjacent pockets | This paper | EMD-24087; PDB-7MZA |
| minimal TRPV1, 2 bound RTX in opposite pockets | This paper | EMD-24091; PDB-7MZE |
| minimal TRPV1, 3 bound RTX and 1 perturbed PI | This paper | EMD-24088; PDB-7MZB |
| minimal TRPV1, 4 fully engaged RTX C1 | This paper | EMD-24089; PDB-7MZC |
| minimal TRPV1, 4 fully engaged RTX C2 | This paper | EMD-24090; PDB-7MZD |
| full-length TRPV1, apo, neutral pH | This paper | EMD-23128; PDB-7L2H |
| full-length TRPV1, DkTx/RTX | This paper | EMD-23133; PDB-7L2M |
| full-length TRPV1, RTX C1 | This paper | EMD-23134; PDB-7L2N |
| full-length TRPV1, RTX O1 | This paper | EMD-23132; PDB-7L2L |
| full-length TRPV1, RTX C2 | This paper | EMD-24083; PDB-7MZ5) |
| full-length TRPV1, pH6a | This paper | EMD-23129; PDB-7L2I |
| full-length TRPV1, pH6b | This paper | EMD-23131; PDB-7L2K |
| full-length TRPV1, pH6c | This paper | EMD-23130; PDB-7L2J |
| full-length TRPV1, pH5.5-RTX | This paper | EMD-23135; PDB-7L2O |
| TRPV1 in complex with DkTx and RTX | Gao et al., 2016 | PDB: 5irx |
| NavAb N49K/L109A/M116V/G94C/Q150C disulfide crosslinked mutant | Wisedchaisri et al., 2019 | PDB: 6P6W |
| NavAb V100C/Q150C disulfide crosslinked mutant | Wisedchaisri et al., 2019 | PDB: 6P6Y |
| Potassium Channel KcsA-Fab complex | Zhou et al., 2001 | PDB: 1k4c |
| Recombinant DNA | ||
| Plasmid: pET28a_MSP2N2 | (Grinkova et al., 2010) |
http://n2t.net/addgene:29520; RRID:Addgene_29520 |
| Plasmid: pBH_His_DkTx | Bohlen et al., 2010 | N/A |
| Plasmid: pFastBac1-CMV_His-MBP_mini-TRPV1 | This paper | N/A |
| Plasmid: pFastBac1-CMV_His-MBP_FL-TRPV1 | This paper | N/A |
| Software and algorithms | ||
| SerialEM | Mastronarde, 2005 |
http://bio3d.colorado.edu/SerialEM/; RRID:SCR_017293 |
| MotionCor2 | Zheng et al., 2017 |
https://msg.ucsf.edu/software; RRID:SCR_016499 |
| GCTF | Zhang, 2016 |
https://cam.ac.uk/kzha; RRID:SCR_016500 |
| Relion 3.0 | (Zivanov et al., 2018) |
https://cam.ac.uk/relion; RRID:SCR_016274 |
| Relion 3.1 | (Scheres, 2020) |
https://cam.ac.uk/relion; RRID:SCR_016274 |
| cryoSPARC | (Punjani et al., 2017) |
https://cryosparc.com; RRID:SCR_016501 |
| cisTEM | Grant et al., 2018 |
https://cistem.org/; RRID:SCR_016502 |
| PyEM | Daniel Asarnow, Yifan Cheng Lab |
https://github.com/asarnow/pyem; https://doi.org/10.5281/zenodo.3576630 |
| UCSF Chimera | Pettersen et al., 2004 |
https://www.cgl.ucsf.edu/chimera; RRID:SCR_004097 |
| COOT | Emsley et al., 2010 |
https://www2.mrclmb.cam.ac.uk/personal/pemsley/coot/; RRID:SCR_014222 |
| PHENIX | Adams et al., 2010 |
http://www.phenixonline.org; RRID:SCR_014224 |
| ISOLDE | Croll, 2018 | https://isolde.cimr.cam.ac.uk/ |
| Prism 7 | (GraphPad Software, San Diego, CA). |
https://www.graphpad.com/scientificsoftware/prism/; RRID:SCR_002798 |
| Other | ||
| Amylose Resin | NEB | Cat#E8021S |
| QUANTIFOIL R1.2/1.3 300 mesh Au holey carbon grids | quantifoil | https://www.quantifoil.com/ |