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. 2021 Jul 23;81(19):5047–5059. doi: 10.1158/0008-5472.CAN-21-0691

Figure 1.

Figure 1.

Point mutations in the endogenous Alk locus and neuroblastoma formation. A, Diagram of the two ALK mutations introduced into the endogenous Alk locus. B, Experimental design of the longitudinal study for animals reported herein. C, Incidence and survival curves comparing tumor formation of AlkF1178L; TH-MYCN or AlkY1282S; TH-MYCN mice. Representative image of an AlkF1178L; TH-MYCN mouse with a large abdominal neuroblastoma. D, Clustered heat map of “signature” gene expression comparing murine neuroblastoma (NB) models and other human solid tumors. E, GD2 expression in CD45− tumor cells. Purple and green histograms show GD2 expression from two individual tumors compared with isotype control (gray histogram). F, CD276 (B7-H3) expression in tumor cells. Purple and green histograms show CD276 expression from CD45− cells from two individual tumors compared with isotype control (gray histogram). G, Frequency of PD-L1– and PD-L2–expressing cells in CD45+ or CD45− intratumoral populations. In C, data were analyzed by the log-rank test. Criteria for scoring tumor incidence and inclusion and exclusion criteria are described in Materials and Methods. In D, the complete transcriptional signature is available in Supplementary Table S3 and available in full using accession numbers GSE12460, GSE13136, GSE16237, GSE37372, GSE29683, E-TABM-1202, GSE27516, GSE98763, and GSE27516. Red font, canonical neuroblastoma-associated mRNAs.