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. 2021 Sep 29;14:4987–5000. doi: 10.2147/JIR.S329234

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© 2021 Hong et al.

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Serum levels of syndecan-2 are not detected in an TNBS-induced colitis mice. (A) Mouse tissues from acute TNBS model were stained with H&E or immunostained with anti-syndecan-2 and -MMP-7 antibodies (n=3~4 mice per group). Scale bars: 50 μM. (B) The associations between the syndecan-2 expression area and the inflammatory area in acute TNBS model was analyzed using correlation analysis, as applied by the ANOVA test program of SPSS (version 20.0). (C) Mice serum were collected and analyzed by slot blotting with anti-syndecan-2 polyclonal antibody. Quantitative analysis of shed syndecan-2 levels in mice serum were performed using box-whisker plot. Shed syndecan-2 level in vehicle mice was used as a control. *p < 0.05.