TABLE 1.

Configuration of stacking gel and separation gel.

Ingredients	Stacking gel	10% Separation gel
Double distilled water	2.6 × 103	3 × 103
30% polyacrylamide liquid	0.64 × 103	3 × 103
1.0 mol/L tromethamine (Tris, pH8.8)	2.3 × 103	2.3 × 103
10% sodium dodecyl sulfate (SDS)	0.03 × 103	0.1 × 103
10% ammonium persulfate	0.04 × 103	0.1 × 103
Tetramethylethylenediamine (TEMED)	0.004 × 103	0.004 × 103