FIGURE 4.

Linc00284 directly targets miR-205-3p. (A) Schematic representation of direct binding sites between Linc00284 and miR-205-3p. Red indicates the predicted binding sites between Linc00284 and miR-205-3p. Green indicates the mutant sequence of Linc00284. (B) Dual-luciferase reporter assay confirmed direct interaction between miR-205-3p and Linc00284. (C) RT-qPCR revealed the significant downregulation of miR-205-3p in lung cancer tissues. (D–G) miR-205-3p expression was associated with (D) TNM stage, (E) recurrence, (F) metastasis, and (G) overall survival of patients with lung cancer. (H) Pearson’s correlation analysis indicated that miR-205-3p expression was negatively correlated with the Linc00284 level in tumor tissues. (I) miR-205-3p expression was significantly upregulated in A549, H1975, and H460 lung cancer cell lines. (J) miR-205-3p was mainly located in the cytoplasm of lung cancer cells. Data are expressed as the means ± SD for triplicate independent experiments. The expression of miR-205-3p in 43 lung cancer tissues and paired adjacent non-cancerous tissues was analyzed using paired t-test. Multiple comparisons were analyzed using one-way ANOVA followed by Tukey’s test. Kaplan–Meier analysis was used for survival analysis. Correlation analysis was assessed using Pearson’s correlation analysis. Descriptive statistics were used to analyze the expression of miR-205-3p in the cytoplasm and in the nucleus of LC cells. *P < 0.05, **P < 0.01, and ***P < 0.001, compared with the matched control group. NS, not significant. N, adjacent normal tissues; T, tumor tissues; R, recurrence; M, metastasis.