Table 1.
Strains and plasmids used in the present work.
| Plasmids | Description | Source or reference | |
|---|---|---|---|
| pET21c | T7 promoter, pBR322 ori, AmpR | Novagen | |
| pRSFDuet-1 | Double T7 promoters, pBR322 ori, KanR | Novagen | |
| pCDFDuet-1 | Double T7 promoters, CloDF13 ori, SmR | Novagen | |
| pYH51 | pCDF-4CL-PcCHS-CHI | pCDF-Duet-1 carrying 4CL, PcCHS, and CHI | Li et al. (2019) |
| pYH55 | pCDF-4CL-PAL-PcCHS-CHI | pCDF-Duet-1 carrying PAL, 4CL, PcCHS, and CHI | Li et al. (2019) |
| pYH70 | pCDF-4CL-PcCHS-GuCHR-CHI | pCDF-Duet-1 carrying 4CL, GuCHR, PcCHS, and CHI | This study |
| pYH88 | pCDF-4CL-PAL-PcCHS-GuCHR-CHI | pCDF-Duet-1 carrying 4CL, PAL, PcCHS, GuCHR, and CHI | This study |
| pYH89 | pCDF-4CL-PAL-GuCHS-GuCHR-CHI | pCDF-Duet-1carrying 4CL, PAL, GuCHS, GuCHR, and CHI | This study |
| pYH102 | pCDF-4CL-PAL-AtCHS-GuCHR-CHI | pCDF-Duet-1 carrying 4CL, PAL, AtCHS, GuCHR, and CHI | This study |
| pYH137 | pCDF-4CL-PAL-PcCHS-GmCHR5-CHI | pCDF-Duet-1 carrying 4CL, PAL, AtCHS, GmCHR5, and CHI | This study |
| pYH138 | pCDF-4CL-PAL-GuCHS-GmCHR5-CHI | pCDF-Duet-1 carrying 4CL, PAL, GuCHS, GmCHR5, and CHI | This study |
| pYH139 | pCDF-4CL-PAL-AtCHS-GmCHR5-CHI | pCDF-Duet-1 carrying 4CL, PAL, AtCHS, GmCHR, and CHI | This study |
| pYH140 | pCDF-4CL-PAL-GmCHS7-GmCHR5-CHI | pCDF-Duet-1 carrying 4CL, PAL, GmCHS7, GmCHR5, and CHI | This study |
| pYH141 | pCDF-4CL-PAL-GmCHS7-GuCHR-CHI | pCDF-Duet-1 carrying 4CL, PAL, GmCHS, GuCHR, and CHI | This study |
| pYH149 | pCDF-4CL-PAL-GmCHS7::PDZlig-GuCHR::SH3lig-CHI::GBDlig | pCDF-Duet-1 carrying 4CL, PAL, GmCHS7::PDZlig, GuCHR::SH3lig, and CHI::GBDlig | This study |
| pYH152 | pCDF-4CL-PAL-GmCHS7::GuCHR-CHI | pCDF-Duet-1 carrying 4CL, PAL, GmCHS7::GuCHR, and CHI | This study |
| pYH153 | pCDF-4CL-PAL-GmCHS7::PDZlig-GuCHR::PDZ-CHI | pCDF-Duet-1 carrying 4CL, PAL, GmCHS7::PDZlig, GuCHR::PDZ, and CHI | This study |
| pYH17 | pET28a-FNS I | pET28a carrying FNS I | Li et al. (2019) |
| pYH164 | pRSF-17αtrGmIFS::CrCPR-GmHID | pRSFDuet-1 carrying 17αtrGmIFS::CrCPR, and GmHID | This study |
| Strains | |||
| E. coli BL21(DE3) | |||
| DHF-1 | E. coli BL21(DE3) harboring pYH141 and pYH17 | This study | |
| DHF-2 | E. coli BL21(DE3) harboring pYH153 and pYH17 | This study | |
| DHF-3 | E. coli BL21(DE3) harboring pYH149, pYH117, and pYH17 | This study | |
| DAI-1 | E. coli BL21(DE3) harboring pYH141, and pYH164 | This study | |
| DAI-2 | E. coli BL21(DE3) harboring pYH153, and pYH164 | This study | |
| DAI-3 | E. coli BL21(DE3) harboring pYH149, pYH117 and pYH164 | This study | |