Skip to main content
. 2021 Jul 1;12(4):1433–1455. doi: 10.1016/j.jcmgh.2021.06.020

Figure 2.

Figure 2

Identification of the stemness markers in the proliferating cells after ANKRD22 deletion. (A and B) Effects of Ankrd22 knockout on the percentages of Lgr5+, Mist1+, Sox2+, Sox9+, Troy+, Cd44+, Cd133+, Cd166+, Ssea1+, and Ssea4+ cells after gastric mucosal injury detected by FCM. Rat IgG2b was used as isotype control. (C–E) Effects of Ankrd22 knockout on Lgr5, Mist1, Sox2, Sox9, Cd44, Cd133, Cd166, Ssea1, and Ssea4 in organoid-enriched primary mouse gastric EPCs detected by qRT-PCR. The organoids were derived from the whole-mouse gastric tissue as well as the corpus and antrum. The data from the Ankrd22+/+ group were normalized to 1.0. TUBB was used as an internal reference. (A–E) Ankrd22+/+ and Ankrd22-/- mice were subjected to subsequent detection at 24 hours after intragastric administration of EtOH/HCl or saline solution (Ctrl) (n = 3). Data are presented as means ± SD and analyzed by the Student t test. ∗P < .05 and ∗∗P < .01. APC-A, Allophycocyanin Area; FITC-A, Fluorescein Isothiocyanate Area; FSC-A, Forward Scatter Area; PE-A, Phycoerythrin Area.